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Such unique physicochemical properties draw global attention of scientists to study potential risks and adverse effects of nanoparticles in the environment. Their toxicity has pronounced effects and consequences for plants and ultimately the whole ecosystem. Plants growing in nanomaterials-polluted sites may exhibit altered metabolism, growth reduction, and lower biomass production. Nanoparticles can adhere to plant roots and exert physicochemical toxicity and subsequently cell death in plants. On the other hand, plants have developed various defense mechanisms against this induced toxicity. This books discusses recent findings as well as several unresolved issues and challengesregarding the interaction and biological effects of nanoparticles. 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