Methods in Molecular Biology

This detailed volume explores techniques for performing calcium signaling in diverse cell types, alongside detailed methods for data analysis. Beginning with chapters on cellular assays to assess calcium signaling, the book continues with sections on imaging methods, methods to assess calcium within primary cilia, the endoplasmic reticulum, and the mitochondria, as well as tissue-specific calcium signaling methods, including the establishment of primary cell or tissue models and measuring calcium in whole organisms such as Drosophila. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Calcium Signaling: Methods and Protocols serves as an ideal guide for laboratories of all types seeking to measure calcium robustly.