Methods in Molecular Biology

This volume provides readers with an overview of lentiviral vector applications and includes detailed laboratory protocols relevant to neuroscience research. The chapters are organized into five sections: Section I provides an overview of lentiviral vector properties, including practical methods for vector preparation and delivery into the brains of rodents and non-human primates, as well as delivery into non-human primate embryos for transgenic animal production. Sections II to IV explore basic neuroscience covering anatomical, molecular and developmental, and behavioral and physiological neuroscience. Section V focuses on clinical neuroscience, offering protocols related to gene therapy for motor neuron and ocular diseases. Written in the highly successful Methods in Molecular Biology series format, each chapter includes an introduction to the topic, a list of required materials and reagents, step-by-step protocols that are easy to reproduce, and expert tips for troubleshooting and avoiding common pitfalls.Cutting-edge and thorough, Lentiviral Vectors: Application for Basic and Clinical Neuroscience is a valuable resource for any researcher looking to learn more about this important and advancing field.