Methods in Molecular Biology

This second volume details new and updated methods on biology of various cellular processes and agronomic traits and come up with better strategies to improve legume crops. Chapters guide readers through legume genomic resources, legume pangenome and organelle genome construction, transcriptome analysis, DNA methylation analysis, double-digest restriction site-associated DNA sequencing; target enrichment sequencing via probe capture, genomic selection and transformation methods; prediction of long non-coding RNAs and secondary structures, genome-wide mining of disease resistance gene analogs; genome editing, and bioactive compound and phosphoproteome analysis. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Legume Genomics: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.