Methods in Molecular Biology

This detailed volume presents methods for investigating mitotic spindle assembly, chromosome segregation, and cytokinesis. Split into six parts, the book examines advanced microscopes, spatiotemporal manipulation of the spindle and target molecules, quantitative live imaging, screening, centrosome manipulation and purification, and spindle positioning in synthetic or complicated contexts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding know pitfalls. Authoritative and up-to-date, The Mitotic Spindle: Methods and Protocols serves as an ideal guide for researchers seeking a better system-level understanding of the mitotic spindle. Chapters 4, 12, 17, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.