Methods in Molecular Biology

This volume covers the state-of-art lineage tracing methods, to record these recent multi-front rapid progresses and promote development of further powerful tools. Chapters explores a broad spectrum of techniques that have been developed and refined over the years, offering a detailed exposition of their principles, applications, and limitations. Additional chapters cover classic methods, such as genetic lineage tracing, clonal analysis, single-cell RNA sequencing, and live-cell imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lineage Tracing: Methods and Protocols aims to serve as a valuable resource, fostering new discoveries and advancements in the exciting field of cell lineage tracing.