Nutrient Sensing in Eukaryotes

This detailed volume explores various methods through which nutrient sensing can be studied, particularly in multicellular eukaryotes. Beginning with a section on the monitoring of cellular energy status by sensing of adenine nucleotides, the book continues by delving into numerous techniques for researching both intracellular and extracellular nutrient sensing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nutrient Sensing in Eukaryotes serves as an ideal guide for researchers aiming to develop related or novel methods to study the sensing of nutrients in their own systems. Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.